ALEXIS Biochemicals: Monoclonal Antibody to BAP31 (CC-1)

ALX-804-812

Revised 20-Jun-08

Monoclonal Antibody to BAP31 (CC-1)
SYNONYMS	anti-B Cell Receptor-associated Protein BAP31 MAb (CC-1) anti-BCR-associated Protein BAP31 MAb (CC-1) anti-p28 BAP31 MAb (CC-1) anti-CDM Protein MAb (CC-1) anti-6C6-AG Tumor-associated Antigen MAb (CC-1) anti-DXS1357E MAb (CC-1)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	B Cell Receptor [BCR] & Co-Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-812-C100		100 µg	320.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Goat Sheep Monkey Others
CLONE:	CC-1
ISOTYPE:	Rat IgG2a
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	0.5mg/ml
PURITY:	>95% (SDS-PAGE)
FORMULATION:	Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN:	Solubilized membranes of the IMR-32 human neuroblastoma cell line.
SPECIFICITY:	Recognizes an epitope (aa 230-246) encompassing a caspase recognition site and the ER-homing motif (KKEE), present at the C-terminus of human, primate, bovine and hamster BAP31. Does not recognize mouse and rat BAP31.
APPLICATION:	ELISA (capture) Immunocytochemistry (1:1'000-1:10'000; works on endogenous) Immunohistochemistry (frozen sections (1:1'000-1:10'000); works on endogenous) Immunoprecipitation Western Blot (1:1'000-1:6'000; works on endogenous).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

Endoplasmic reticulum membrane-sorting protein of lymphocytes (BAP31) is highly expressed in neurons and discrete endocrine cells: H.A. Manley and V.A. Lennon; J. Histochem. Cytochem. 49, 1235 (2001) Abstract; Full Text
Association of BAP31 with CD11b/CD18. Potential role in intracellular trafficking of CD11b/CD18 in neutrophils: K. Zen, et al.; J. Biol. Chem. 279, 44924 (2004) Abstract; Full Text
Bap31 enhances the endoplasmic reticulum export and quality control of human class I MHC molecules: J.J. Ladasky, et al.; J. Immunol. 177, 6172 (2006) Abstract

General Information

BAP31 (B cell receptor-associated protein 31) is an integral protein of the ER membrane that forms a large hetero-oligomeric complex with the related BAP29. The cytosolic domain of BAP31 contains two identical caspase recognition sites that are preferentially cleaved by initiator caspases, including caspase-8. After activation of cell surface death receptors, human BAP31 is cleaved into a membrane-embedded fragment called p20, which induces apoptosis when expressed ectopically. The p20 fragment can mediate Ca²⁺-dependent apoptotic cross-talk between the ER and mitochondria, stimulating cytochrome c release. In addition to its role in apoptosis, BAP31 is a component of the ER quality control compartment and functions as a cargo receptor for ER export of transmembrane proteins such as cellubrevin, MHC class I molecules, and cytochrome P450 2C2.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Caspase-resistant BAP31 inhibits fas-mediated apoptotic membrane fragmentation and release of cytochrome c from mitochondria: M. Nguyen, et al.; Mol. Cell. Biol. 20, 6731 (2000) Abstract; Full Text
Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol: D.G. Breckenridge, et al.; J. Cell. Biol. 160, 1115 (2003) Abstract; Full Text
Bap31 Is an Itinerant Protein that Moves between the Peripheral ER and a Juxtanuclear Compartment Related to ER-associated Degradation: Y. Wakana, et al.; Mol. Biol. Cell 19, 1825 (2008) Abstract; Full Text

Further Categories Containing This Product:

Endoplasmatic Reticulum Stress • ER-Golgi Intermediate Compartment [ERGIC] / Related Products • Monoclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products

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