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ALX-804-196 Revised 27-Aug-08
Monoclonal Antibody to Ceramide (MID 15B4)
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PRODUCT LINE Neurobiology
PRODUCT CATEGORY Ceramides / Cerebrosides / Sphingolipids / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-196-T050   50 tests 315.00 USD Add To Cart
Product Specification
CLONE: MID 15B4
ISOTYPE: Mouse IgM
SOURCE/HOST: Purified from ascites by gel filtration on sephacryl S-300.
QUANTITY: 1ml (50 tests). Suggested amount: 20µl/test.
CONCENTRATION: 200µg/ml
PURITY: ≥95%
FORMULATION: Liquid. In PBS, pH 7.2, containing 0.5M sodium chloride, 0.1% BSA and 0.09% sodium azide.
IMMUNOGEN: Ceramide (sphingosine-[trans-D-erythro-2-amino-4-octadecene-1,3-diol]) conjugated to BSA.
SPECIFICITY: Recognizes C16- and C24-ceramide, dihydroceramide, sphingomyelin and phosphatidylcholine in highly artificial lipid overlay test systems. Under more physiological in vitro and in vivo conditions highly specific for ceramide and does not cross-react with sphingomyelin, cholesterol or other phospholipids (see lit. ref. [1], [2], [3]).
APPLICATION: ELISA (1:10)
Flow Cytometry (see [2])
Immunocytochemistry (see [11,12])
Immunohistochemistry (1:10)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Product Specific Literature References
[1] Structural determinants of sphingolipid recognition by commercially available anti-ceramide antibodies: L.A. Cowart, et al.; J. Lipid Res. 43, 2042 (2002) Abstract; Full Text
[2] CD95 Signaling Via Ceramide-rich Membrane Rafts: H. Grassme, et al.; J. Biol. Chem. 276, 20589 (2002) Abstract; Full Text
[3] Ceramide-Rich Membrane Rafts Mediate CD40 Clustering: H. Grassme, et al.; J. Immunol. 168, 298 (2002) Abstract; Full Text
[4] Ceramide inhibits the potassium channel Kv1.3 by the formation of membrane platforms: J. Bock, et al.; BBRC 305, 890 (2003) Abstract
[5] Host defense against Pseudomonas aeruginosa requires ceramide-rich membrane rafts: H. Grassme, et al.; Nat. Med. 9, 322 (2003) Abstract
[6] Regulation of cell death in mitotic neural progenitor cells by asymmetric distribution of prostate apoptosis response 4 (PAR-4) and simultaneous elevation of endogenous ceramide: E. Bieberich, et al.; J. Cell Biol. 162, 469 (2003) Abstract; Full Text
[7] PAF-mediated pulmonary edema: a new role for acid sphingomyelinase and ceramide: R. Goggel, et al.; Nat. Med 10, 155 (2004) Abstract
[8] Study on ceramide expression and DNA content in patients with healthy mucosa, leukoplakia, and carcinoma of the larynx: F.L. Chi, et al.; Arch. Otolaryngol. Head Neck Surg. 130, 307 (2004) Abstract
[9] Involvement of ceramide in hyperosmotic shock-induced death of erythrocytes: K.S. Lang, et al.; Cell Death Differ. 11, 231 (2004) Abstract
[10] Inhibition of erythrocyte phosphatidylserine exposure by urea and Cl: K.S. Lang, et al.; Am. J. Physiol. Lung Cell Mol. Physiol. 286, F1046 (2004) Abstract
[11] Clustering of CD40 ligand is required to form a functional contact with CD40: H. Grassme, et al.; J. Biol. Chem. 277, 30289 (2002) Abstract
[12] Caspase-dependent and -independent activation of acid sphingomyelinase signaling: J.A. Rotolo, et al.; J. Biol. Chem. 280, 26425 (2005) Abstract; Full Text
Further Categories Containing This Product:
Autophagy Other ProductsMonoclonal Antibodies
 
 

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