ALEXIS Biochemicals: Monoclonal Antibody to PTX3 (MNB4)

ALX-804-464

Revised 06-Nov-07

Monoclonal Antibody to PTX3 (MNB4)
SYNONYMS	anti-Pentraxin 3 MAb (MNB4) anti-TSG14 MAb (MNB4) anti-TNF Stimulated Gene-14 MAb (MNB4)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	Pentraxin3 [PTX3] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-464-C100		100 µg	350.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Rat
CLONE:	MNB4
ISOTYPE:	Rat IgG2a
CONCENTRATION:	1mg/ml
FORMULATION:	Liquid. In PBS containing 0.1% sodium azide.
IMMUNOGEN:	Recombinant human PTX3 (pentraxin 3).
SPECIFICITY:	Recognizes the N-terminus of human and rat PTX3.
APPLICATION:	ELISA (capture - excellent) Immunohistochemistry (frozen sections (1µg/ml)) Immunoprecipitation Western Blot
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

Multimer formation and ligand recognition by the long pentraxin PTX3. Similarities and differences with the short pentraxins C-reactive protein and serum amyloid P component: B. Bottazzi, et al.; J. Biol. Chem. 272, 32817 (1997) Abstract
PTX3, A Prototypical Long Pentraxin, is an Early Indicator of Acute Myocardial Infarction in Humans: G. Peri, et al.; Circulation 102, 636 (2000) Abstract; Full Text
Production of the Long Pentraxin PTX3 in Advanced Atherosclerotic Plaques: M.S. Rolph, et al.; Arterioscler. Thromb. Vasc. Biol. 22, e10 (2002) Abstract
The long Pentraxin Ptx3 is synthesized in IgA glomerulonephritis and Activates Mesangial Cells: B. Bussolati, et al.; J. Immunol. 170, 1466 (2003) Abstract
Pentraxin 3 is elevated in haemodialysis patients and is associated with cardiovascular disease: M. Boehme, et al.; Nephrol. Dial. Transplant. 22, 2224 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Sandwich ELISA for PTX3

- 96 well-ELISA plates (Nunc MaxiSorp 446612) were coated with 100µl of MAb to PTX3 (human) (MNB4) (Prod. No. ALX-804-464), 700ng/ml in 100µl coating buffer (15mM carbonate buffer pH 9.6) and incubated overnight at 4°C.
- After incubation, the plates were washed three times with 300µl/well of washing buffer (PBS + 0.05% Tween 20), and then 300µl of 5% dry milk in washing buffer were added to block non-specific binding.
- The plates were incubated for 2 hours at room temperature and then washed three times with washing buffer.
- 50µl (in duplicate) recombinant human PTX3 standard (Prod. No. ALX-201-124) (75pg/ml to 2.4ng/ml ), cell supernatants or biological fluids diluted in RPMI 1640 + 2% BSA were added and then incubated for 2 hours at 37°C.
- The plates were washed 5 x with washing buffer, and then 100µl of PAb to PTX3 (human) (Biotin) (Prod. No. ALX-210-365) 25ng/ml in washing buffer were added to each well.
- The plates were incubated for 1 hour at 37°C and then washed five times with 300µl of washing buffer.
- 100µl/well streptavidin-horseradish peroxidase diluted 1:4’000 were added and the plates incubated for 1 hour at room temperature.
- After incubation the plates were washed 5 x and 100µl of chromogen substrate ABTS were added. Plates were read after 15 minutes at 405nm in an automatic ELISA reader.